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Fishing bot 3.3 5a3/11/2024 Western blot was utilized for detection of TLR4 protein expression. Quantitative real time polymerase chain reaction was used to detect IL-6, IL-1β and TNF-α messenger RNA levels. Concentrations of tumor necrosis factor (TNF)-α, Interleukin (IL)-6 and IL-1β in the hippocampus were assessed via enzyme linked immunosorbent assay (ELISA). Microglia activation was detected by Iba1 immunofluorescence, and neuronal injury was detected by Nissl staining. Tail suspension test (TST), forced swimming test (FST) and sucrose preference test were performed to monitor the depression behavior of mice. The mouse model of depression was established through chronic unpredictable mild stress (CUMS), the mice were intervened with n-3 PUFAs, and then the expression of toll-like receptor 4 (TLR4) was stimulated with lipopolysaccharides (LPS). N-3 polyunsaturated fatty acids (PUFAs) are believed to be implicated in the pathogenesis of many inflammation-related diseases, including depression.
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